› 2gwj

X-ray diffraction
1.9Å resolution

SpvB ADP-ribosylated actin: hexagonal crystal form

Released:
DOI: 10.2210/pdb2gwj/pdb
PDB entry 2gwj coloured by chain, front view.
PDB entry 2gwj coloured by chain, side view.
PDB entry 2gwj coloured by chain, top view.
Source organism: Oryctolagus cuniculus
Primary publication:
A steric antagonism of actin polymerization by a salmonella virulence protein.
Margarit SM, Davidson W, Frego L, Stebbins CE
Structure 14 1219-29 (2006)
PMID: 16905096

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159302 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Actin, alpha skeletal muscle Chain: A
Molecule details ›
Chain: A
Length: 371 amino acids
Theoretical weight: 41.39 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P68135 (Residues: 7-377; Coverage: 98%)
Gene names: ACTA, ACTA1
Sequence domains: Actin
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand ATP 1 x ATP
1 modified residue:
Ligand HIC 1 x HIC

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9A
Spacegroup: P3221
Unit cell:
a: 96.361Å b: 96.361Å c: 97.41Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.153 0.152 0.184

Quick links

Citations

15 review citations

Toward a unified nomenclature for mammalian ADP-ribosyltransferases.
Hottiger et al. (2010)
14 more

2 mentions without citation

Speaking the host language: how Salmonella effector proteins manipulate the host.
Pillay et al. (2023)
1 more