› 2gwr

X-ray diffraction
2.1Å resolution

Crystal structure of the response regulator protein mtrA from Mycobacterium Tuberculosis

Released:
DOI: 10.2210/pdb2gwr/pdb
PDB entry 2gwr coloured by chain, front view.
PDB entry 2gwr coloured by chain, side view.
PDB entry 2gwr coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Domain orientation in the inactive response regulator Mycobacterium tuberculosis MtrA provides a barrier to activation.
Friedland N, Mack TR, Yu M, Hung LW, Terwilliger TC, Waldo GS, Stock AM
Biochemistry 46 6733-43 (2007)
PMID: 17511470

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161504 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-binding response regulator MtrA Chain: A
Molecule details ›
Chain: A
Length: 238 amino acids
Theoretical weight: 26.85 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WGM7 (Residues: 1-228; Coverage: 100%)
Gene names: MTCY20B11.21c, Rv3246c, mtrA
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand GOL 2 x GOL
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P212121
Unit cell:
a: 38.916Å b: 56.598Å c: 135.069Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.204 0.244
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Adaptation to environmental stimuli within the host: two-component signal transduction systems of Mycobacterium tuberculosis.
Bretl et al. (2011)
6 more

14 mentions without citation

Molecular strategies for phosphorylation-mediated regulation of response regulator activity.
Gao et al. (2010)
13 more