› 2gx0

X-ray diffraction
1.9Å resolution

Crystal structural and functional analysis of GFP-like fluorescent protein

Released:
DOI: 10.2210/pdb2gx0/pdb
PDB entry 2gx0 coloured by chain, front view.
PDB entry 2gx0 coloured by chain, side view.
PDB entry 2gx0 coloured by chain, top view.
Source organism: Echinophyllia sp. SC22
Primary publication:
Structural characterization of the photoswitchable fluorescent protein Dronpa-C62S.
Nam KH, Kwon OY, Sugiyama K, Lee WH, Kim YK, Song HK, Kim EE, Park SY, Jeon H, Hwang KY
Biochem Biophys Res Commun 354 962-7 (2007)
PMID: 17276392

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-178339 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fluorescent protein Dronpa Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 241 amino acids
Theoretical weight: 27.61 KDa
Source organism: Echinophyllia sp. SC22
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5TLG6 (Residues: 2-224; Coverage: 100%)
Gene name: Dronpa
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand GYC 4 x GYC

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 4A, PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P212121
Unit cell:
a: 73.595Å b: 103.595Å c: 122.326Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.178 0.211
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 citation in other articles

Light-dependent regulation of structural flexibility in a photochromic fluorescent protein.
Mizuno et al. (2008)
9 more

1 mention without citation

Crystallization and preliminary X-ray analysis of a monomeric mutant of Azami-Green (mAG), an Aequorea victoria green fluorescent protein-like green-emitting fluorescent protein from the stony coral Galaxea fascicularis.
Ebisawa et al. (2009)