› 2gx8

X-ray diffraction
2.2Å resolution

The Crystal Structure of Bacillus cereus protein related to NIF3

Released:
DOI: 10.2210/pdb2gx8/pdb
PDB entry 2gx8 coloured by chain, front view.
PDB entry 2gx8 coloured by chain, side view.
PDB entry 2gx8 coloured by chain, top view.
Source organism: Bacillus cereus ATCC 14579
Primary publication:
The 2.2 A resolution crystal structure of Bacillus cereus Nif3-family protein YqfO reveals a conserved dimetal-binding motif and a regulatory domain.
Godsey MH, Minasov G, Shuvalova L, Brunzelle JS, Vorontsov II, Collart FR, Anderson WF
Protein Sci 16 1285-93 (2007)
PMID: 17586767

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182448 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTP cyclohydrolase 1 type 2 homolog Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 397 amino acids
Theoretical weight: 43.65 KDa
Source organism: Bacillus cereus ATCC 14579
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q818H0 (Residues: 1-373; Coverage: 100%)
Gene name: BC_4286
Sequence domains: Duf34/NIF3 (NGG1p interacting factor 3)
Structure domains: Alpha-Beta Plaits

Ligands and Environments

3 bound ligands:
Ligand ZN 6 x ZN
Ligand EPE 5 x EPE
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B
Spacegroup: P3221
Unit cell:
a: 95.036Å b: 95.036Å c: 260.732Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.199 0.196 0.261
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 citation in other articles

Crystal structure of an apo form of Shigella flexneri ArsH protein with an NADPH-dependent FMN reductase activity.
Vorontsov et al. (2007)
8 more

4 mentions without citation

The 2.2 A resolution crystal structure of Bacillus cereus Nif3-family protein YqfO reveals a conserved dimetal-binding motif and a regulatory domain.
Godsey et al. (2007)
3 more