› 2gz6

X-ray diffraction
2Å resolution

Crystal Structure Of Anabaena sp. CH1 N-acetyl-D-glucosamine 2-epimerase At 2.0 A

Released:
DOI: 10.2210/pdb2gz6/pdb
PDB entry 2gz6 coloured by chain, front view.
PDB entry 2gz6 coloured by chain, side view.
PDB entry 2gz6 coloured by chain, top view.
Source organism: Anabaena sp. CH1
Primary publication:
The central cavity from the (alpha/alpha)6 barrel structure of Anabaena sp. CH1 N-acetyl-D-glucosamine 2-epimerase contains two key histidine residues for reversible conversion.
Lee YC, Wu HM, Chang YN, Wang WC, Hsu WH
J Mol Biol 367 895-908 (2007)
PMID: 17292397

Function and Biology Details

Reaction catalysed: 
D-mannose 6-phosphate = D-fructose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107428 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-acetyl-D-glucosamine 2-epimerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 388 amino acids
Theoretical weight: 45.11 KDa
Source organism: Anabaena sp. CH1
Expression system: Escherichia coli
UniProt:
  • Canonical: A4UA16 (Residues: 1-388; Coverage: 100%)
Gene name: age
Sequence domains: N-acylglucosamine 2-epimerase (GlcNAc 2-epimerase)
Structure domains: Glycosyltransferase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 79.267Å b: 100.027Å c: 104.878Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.165 0.226
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Polyhydroyxalkanoate synthase fusions as a strategy for oriented enzyme immobilisation.
Hooks et al. (2014)
3 more

2 mentions without citation

Functional classification of protein structures by local structure matching in graph representation.
Mills et al. (2018)
1 more