› 2gzb

X-ray diffraction
1.7Å resolution

Bauhinia bauhinioides cruzipain inhibitor (BbCI)

Released:
DOI: 10.2210/pdb2gzb/pdb
PDB entry 2gzb coloured by chain, front view.
PDB entry 2gzb coloured by chain, side view.
PDB entry 2gzb coloured by chain, top view.
Source organism: Bauhinia bauhinioides
Primary publication:
Crystal structure of a novel cysteinless plant Kunitz-type protease inhibitor.
Hansen D, Macedo-Ribeiro S, Veríssimo P, Yoo Im S, Sampaio MU, Oliva ML
Biochem Biophys Res Commun 360 735-40 (2007)
PMID: 17631863

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160665 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Kunitz-type proteinase inhibitor BbCI Chains: A, B
Molecule details ›
Chains: A, B
Length: 166 amino acids
Theoretical weight: 18.32 KDa
Source organism: Bauhinia bauhinioides
Expression system: Escherichia coli
UniProt:
  • Canonical: P83051 (Residues: 1-164; Coverage: 100%)
Sequence domains: Trypsin and protease inhibitor
Structure domains: Trefoil (Acidic Fibroblast Growth Factor, subunit A)

Ligands and Environments

1 bound ligand:
Ligand IOD 10 x IOD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 62.009Å b: 41.299Å c: 63.523Å
α: 90° β: 98.44° γ: 90°
R-values:
R R work R free
0.201 0.199 0.244
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Plant storage proteins with antimicrobial activity: novel insights into plant defense mechanisms.
Cândido Ede et al. (2011)
7 more

4 mentions without citation

The ternary structure of the double-headed arrowhead protease inhibitor API-A complexed with two trypsins reveals a novel reactive site conformation.
Bao et al. (2009)
3 more