› 2gzx

X-ray diffraction
2.2Å resolution

Crystal Structure of the TatD deoxyribonuclease MW0446 from Staphylococcus aureus. Northeast Structural Genomics Consortium Target ZR237.

Released:
DOI: 10.2210/pdb2gzx/pdb
PDB entry 2gzx coloured by chain, front view.
PDB entry 2gzx coloured by chain, side view.
PDB entry 2gzx coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus MW2
Entry authors: Vorobiev SM, Neely H, Seetharaman J, Wang D, Fang Y, Xiao R, Acton T, Montelione GT, Tong L, Hunt JF, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101982 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
putative TatD related DNase Chains: A, B
Molecule details ›
Chains: A, B
Length: 265 amino acids
Theoretical weight: 30.67 KDa
Source organism: Staphylococcus aureus subsp. aureus MW2
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0H3JV42 (Residues: 1-257; Coverage: 100%)
Gene name: MW0446
Sequence domains: TatD related DNase
Structure domains: Metal-dependent hydrolases

Ligands and Environments

1 bound ligand:
Ligand NI 4 x NI
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P21
Unit cell:
a: 46.678Å b: 77.324Å c: 76.129Å
α: 90° β: 98.01° γ: 90°
R-values:
R R work R free
0.258 0.258 0.286
Expression system: Escherichia coli

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