› 2h0r

X-ray diffraction
2.9Å resolution

Structure of the Yeast Nicotinamidase Pnc1p

Released:
DOI: 10.2210/pdb2h0r/pdb
PDB entry 2h0r coloured by chain, front view.
PDB entry 2h0r coloured by chain, side view.
PDB entry 2h0r coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Crystal structure of the yeast nicotinamidase Pnc1p.
Hu G, Taylor AB, McAlister-Henn L, Hart PJ
Arch Biochem Biophys 461 66-75 (2007)
PMID: 17382284

Function and Biology Details

Reaction catalysed: 
Nicotinamide + H(2)O = nicotinate + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156734 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nicotinamidase Chains: A, B, C, D, E, F, G
Molecule details ›
Chains: A, B, C, D, E, F, G
Length: 216 amino acids
Theoretical weight: 25.03 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P53184 (Residues: 1-216; Coverage: 100%)
Gene names: PNC1, YGL037C
Sequence domains: Isochorismatase family
Structure domains: Isochorismatase-like

Ligands and Environments

1 bound ligand:
Ligand ZN 7 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: R3
Unit cell:
a: 302.04Å b: 302.04Å c: 112.12Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.191 0.189 0.22

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Citations

1 review citation

Amide Bond Activation of Biological Molecules.
Mahesh et al. (2018)
16 other articles

8 mentions without citation

Crystal structure of the yeast nicotinamidase Pnc1p.
Hu et al. (2007)
7 more