› 2h0v

X-ray diffraction
2.6Å resolution

Crystal structure of a putative quercetin 2,3-dioxygenase (yxag, bsu39980) from bacillus subtilis at 2.60 A resolution

Released:
DOI: 10.2210/pdb2h0v/pdb
PDB entry 2h0v coloured by chain, front view.
PDB entry 2h0v coloured by chain, side view.
PDB entry 2h0v coloured by chain, top view.
Source organism: Bacillus subtilis
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
Quercetin + O(2) = 2-(3,4-dihydroxybenzoyloxy)-4,6-dihydroxybenzoate + CO + H(+)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154495 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Quercetin 2,3-dioxygenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 338 amino acids
Theoretical weight: 38.13 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P42106 (Residues: 1-337; Coverage: 100%)
Gene names: BSU39980, S14G, qdoI, yxaG
Sequence domains: Cupin domain
Structure domains: Jelly Rolls

Ligands and Environments

4 bound ligands:
Ligand FE 4 x FE
Ligand EDO 18 x EDO
Ligand TLA 2 x TLA
Ligand TRS 2 x TRS
1 modified residue:
Ligand MSE 18 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 97.416Å b: 128.659Å c: 133.889Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.167 0.204
Expression system: Escherichia coli

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