› 2h1n

X-ray diffraction
3Å resolution

3.0 A X-ray structure of putative oligoendopeptidase F: crystals grown by vapor diffusion technique

Released:
DOI: 10.2210/pdb2h1n/pdb
PDB entry 2h1n coloured by chain, front view.
PDB entry 2h1n coloured by chain, side view.
PDB entry 2h1n coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Time-controlled microfluidic seeding in nL-volume droplets to separate nucleation and growth stages of protein crystallization.
Gerdts CJ, Tereshko V, Yadav MK, Dementieva I, Collart F, Joachimiak A, Stevens RC, Kuhn P, Kossiakoff A, Ismagilov RF
Angew Chem Int Ed Engl 45 8156-60 (2006)
PMID: 17099920

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177410 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptidase M3A/M3B catalytic domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 567 amino acids
Theoretical weight: 66.99 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5L1D2 (Residues: 1-564; Coverage: 100%)
Gene name: GK0963
Sequence domains: Peptidase family M3
Structure domains: Neurolysin; domain 3

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand UNL 6 x UNL
1 modified residue:
Ligand MSE 28 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P3121
Unit cell:
a: 119.987Å b: 119.987Å c: 249.747Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.186 0.185 0.211
Expression system: Escherichia coli

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Citations

12 review citations

Droplet microfluidics.
Teh et al. (2008)
11 more

3 mentions without citation

Time-controlled microfluidic seeding in nL-volume droplets to separate nucleation and growth stages of protein crystallization.
Gerdts et al. (2006)
2 more