› 2h1o

X-ray diffraction
3Å resolution

Structure of FitAB bound to IR36 DNA fragment

Released:
DOI: 10.2210/pdb2h1o/pdb
PDB entry 2h1o coloured by chain, front view.
PDB entry 2h1o coloured by chain, side view.
PDB entry 2h1o coloured by chain, top view.
Source organism: Neisseria gonorrhoeae
Primary publication:
Structure of FitAB from Neisseria gonorrhoeae bound to DNA reveals a tetramer of toxin-antitoxin heterodimers containing pin domains and ribbon-helix-helix motifs.
Mattison K, Wilbur JS, So M, Brennan RG
J Biol Chem 281 37942-51 (2006)
PMID: 16982615

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115322 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Toxin FitB Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 143 amino acids
Theoretical weight: 15.92 KDa
Source organism: Neisseria gonorrhoeae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q5F882 (Residues: 1-138; Coverage: 99%)
Gene names: NGO0907, fitB, vapC
Sequence domains: PIN domain
Structure domains: 5'-nuclease
Antitoxin FitA Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 68 amino acids
Theoretical weight: 7.28 KDa
Source organism: Neisseria gonorrhoeae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q5F881 (Residues: 2-69; Coverage: 87%)
Gene names: NGO0908, fitA, vapB
Structure domains: Met repressor-like
IR36-strand 1 Chain: U
Molecule details ›
Chain: U
Length: 36 nucleotides
Theoretical weight: 11.17 KDa
IR36-strand 2 Chain: V
Molecule details ›
Chain: V
Length: 36 nucleotides
Theoretical weight: 11.2 KDa

Ligands and Environments

No bound ligands
1 modified residue:
Ligand 5IU 2 x 5IU

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P21
Unit cell:
a: 75.04Å b: 82.403Å c: 135.503Å
α: 90° β: 94.19° γ: 90°
R-values:
R R work R free
0.269 0.212 0.269
Expression system: Escherichia coli BL21

Quick links

Citations

16 review citations

Toxin-antitoxin systems in bacteria and archaea.
Yamaguchi et al. (2011)
15 more

8 mentions without citation

Structure, Biology, and Therapeutic Application of Toxin-Antitoxin Systems in Pathogenic Bacteria.
Lee et al. (2016)
7 more