2h27

X-ray diffraction
2.3Å resolution

Crystal Structure of Escherichia coli SigmaE Region 4 Bound to its-35 Element DNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-115323 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
ECF RNA polymerase sigma-E factor Chains: A, D
Molecule details ›
Chains: A, D
Length: 73 amino acids
Theoretical weight: 8.44 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AGB6 (Residues: 122-191; Coverage: 37%)
Gene names: JW2557, b2573, rpoE, sigE
Sequence domains: Sigma-70, region 4
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
5'-D(*CP*CP*CP*GP*GP*AP*AP*CP*TP*TP*CP*G)-3' Chains: B, E
Molecule details ›
Chains: B, E
Length: 12 nucleotides
Theoretical weight: 3.62 KDa
Source organism: Escherichia coli K-12
Expression system: Not provided
5'-D(*C*CP*GP*AP*AP*GP*TP*TP*CP*CP*GP*G)-3' Chains: C, F
Molecule details ›
Chains: C, F
Length: 12 nucleotides
Theoretical weight: 3.66 KDa
Source organism: Escherichia coli K-12
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21
Unit cell:
a: 55.009Å b: 68.709Å c: 61.133Å
α: 90° β: 101.25° γ: 90°
R-values:
R R work R free
0.241 0.241 0.253
Expression systems:
  • Escherichia coli
  • Not provided