› 2h28

X-ray diffraction
2.1Å resolution

Crystal structure of YeeU from E. coli. Northeast Structural Genomics target ER304

Released:
DOI: 10.2210/pdb2h28/pdb
PDB entry 2h28 coloured by chain, front view.
PDB entry 2h28 coloured by chain, side view.
PDB entry 2h28 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structures of Phd-Doc, HigA, and YeeU establish multiple evolutionary links between microbial growth-regulating toxin-antitoxin systems.
Arbing MA, Handelman SK, Kuzin AP, Verdon G, Wang C, Su M, Rothenbacher FP, Abashidze M, Liu M, Hurley JM, Xiao R, Acton T, Inouye M, Montelione GT, Woychik NA, Hunt JF
Structure 18 996-1010 (2010)
PMID: 20696400

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160084 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytoskeleton bundling-enhancing antitoxin CbeA Chains: A, B
Molecule details ›
Chains: A, B
Length: 130 amino acids
Theoretical weight: 14.96 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P76364 (Residues: 1-122; Coverage: 100%)
Gene names: JW1986, b2004, cbeA, yeeU
Sequence domains: CbeA_antitoxin, type IV, cytoskeleton bundling-enhancing factor A
Structure domains: PAS domain

Ligands and Environments

3 bound ligands:
Ligand CL 1 x CL
Ligand MG 2 x MG
Ligand GOL 2 x GOL
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P21
Unit cell:
a: 54.216Å b: 45.31Å c: 60.185Å
α: 90° β: 108.79° γ: 90°
R-values:
R R work R free
0.22 0.22 0.253
Expression system: Escherichia coli

Quick links

Citations

22 review citations

Toxin-antitoxin systems in bacteria and archaea.
Yamaguchi et al. (2011)
21 more

3 mentions without citation

α-Conotoxin Peptidomimetics: Probing the Minimal Binding Motif for Effective Analgesia.
Kennedy et al. (2020)
2 more