› 2h31

X-ray diffraction
2.8Å resolution

Crystal structure of human PAICS, a bifunctional carboxylase and synthetase in purine biosynthesis

Released:
DOI: 10.2210/pdb2h31/pdb
PDB entry 2h31 coloured by chain, front view.
PDB entry 2h31 coloured by chain, side view.
PDB entry 2h31 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Octameric structure of the human bifunctional enzyme PAICS in purine biosynthesis.
Li SX, Tong YP, Xie XC, Wang QH, Zhou HN, Han Y, Zhang ZY, Gao W, Li SG, Zhang XC, Bi RC
J Mol Biol 366 1603-14 (2007)
PMID: 17224163

Function and Biology Details

Reactions catalysed: 
5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate = 5-amino-1-(5-phospho-D-ribosyl)imidazole + CO(2)
ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149557 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional phosphoribosylaminoimidazole carboxylase/phosphoribosylaminoimidazole succinocarboxamide synthetase Chain: A
Molecule details ›
Chain: A
Length: 425 amino acids
Theoretical weight: 47.46 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P22234 (Residues: 1-425; Coverage: 100%)
Gene names: ADE2, AIRC, PAICS, PAIS
Sequence domains:
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand CO2 1 x CO2
1 modified residue:
Ligand MSE 7 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P422
Unit cell:
a: 133.666Å b: 133.666Å c: 60.618Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.245 0.241 0.287
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Higher order structures in purine and pyrimidine metabolism.
Chitrakar et al. (2017)
1 more

7 mentions without citation

Structural biology of the purine biosynthetic pathway.
Zhang et al. (2008)
6 more