› 2h3c

Solution NMR

Structural basis for nucleic acid and toxin recognition of the bacterial antitoxin CcdA

Released:
DOI: 10.2210/pdb2h3c/pdb
PDB entry 2h3c coloured by chain, ensemble of 20 models, front view.
PDB entry 2h3c coloured by chain, ensemble of 20 models, side view.
PDB entry 2h3c coloured by chain, ensemble of 20 models, top view.
Source organism: Escherichia coli
Primary publication:
Structural basis for nucleic acid and toxin recognition of the bacterial antitoxin CcdA.
Madl T, Van Melderen L, Mine N, Respondek M, Oberer M, Keller W, Khatai L, Zangger K
J Mol Biol 364 170-85 (2006)
PMID: 17007877

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115325 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Antitoxin CcdA Chains: A, B
Molecule details ›
Chains: A, B
Length: 72 amino acids
Theoretical weight: 8.41 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P62552 (Residues: 1-72; Coverage: 100%)
Gene names: ECOK12F042, H, ccdA, letA
Sequence domains: Post-segregation antitoxin CcdA
5'-D(P*AP*TP*AP*TP*GP*TP*AP*TP*AP*CP*CP*CP*G)-3' Chain: C
Molecule details ›
Chain: C
Length: 13 nucleotides
Theoretical weight: 3.95 KDa
Source organism: Escherichia coli
Expression system: Not provided
5'-D(P*TP*CP*GP*GP*GP*TP*AP*TP*AP*CP*AP*TP*A)-3' Chain: D
Molecule details ›
Chain: D
Length: 13 nucleotides
Theoretical weight: 3.99 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

16 review citations

Toxin-antitoxin systems in bacteria and archaea.
Yamaguchi et al. (2011)
15 more

2 mentions without citation

Wake me when it's over - Bacterial toxin-antitoxin proteins and induced dormancy.
Coussens et al. (2016)
1 more