› 2h3h

X-ray diffraction
1.7Å resolution

Crystal structure of the liganded form of Thermotoga maritima glucose binding protein

Released:
DOI: 10.2210/pdb2h3h/pdb
PDB entry 2h3h coloured by chain, front view.
PDB entry 2h3h coloured by chain, side view.
PDB entry 2h3h coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
Structure-based design of robust glucose biosensors using a Thermotoga maritima periplasmic glucose-binding protein.
Tian Y, Cuneo MJ, Changela A, Höcker B, Beese LS, Hellinga HW
Protein Sci 16 2240-50 (2007)
PMID: 17766373

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194442 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Periplasmic binding protein domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 313 amino acids
Theoretical weight: 33.9 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9WXW9 (Residues: 32-335; Coverage: 91%)
Gene name: TM_0114
Sequence domains: Periplasmic binding protein domain
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand BGC 2 x BGC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: C2
Unit cell:
a: 148.2Å b: 46.06Å c: 118.4Å
α: 90° β: 108.22° γ: 90°
R-values:
R R work R free
0.205 0.203 0.241
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Optical methods for sensing glucose.
Steiner et al. (2011)
5 more

3 mentions without citation

Structure-based design of robust glucose biosensors using a Thermotoga maritima periplasmic glucose-binding protein.
Tian et al. (2007)
2 more