› 2h47

X-ray diffraction
2.6Å resolution

Crystal Structure of an Electron Transfer Complex Between Aromatic Amine Dephydrogenase and Azurin from Alcaligenes Faecalis (Form 1)

Released:
DOI: 10.2210/pdb2h47/pdb
PDB entry 2h47 coloured by chain, front view.
PDB entry 2h47 coloured by chain, side view.
PDB entry 2h47 coloured by chain, top view.
Source organism: Alcaligenes faecalis
Primary publication:
Crystal structure of an electron transfer complex between aromatic amine dehydrogenase and azurin from Alcaligenes faecalis.
Sukumar N, Chen ZW, Ferrari D, Merli A, Rossi GL, Bellamy HD, Chistoserdov A, Davidson VL, Mathews FS
Biochemistry 45 13500-10 (2006)
PMID: 17087503

Function and Biology Details

Reaction catalysed: 
ArCH(2)NH(2) + H(2)O + 2 azurin = ArCHO + NH(3) + 2 reduced azurin
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero dimer
hetero pentamer
hetero tetramer
PDBe Complex ID:
PDB-CPX-132369 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Aralkylamine dehydrogenase heavy chain Chains: A, D, F, H
Molecule details ›
Chains: A, D, F, H
Length: 390 amino acids
Theoretical weight: 42.98 KDa
Source organism: Alcaligenes faecalis
UniProt:
  • Canonical: P84888 (Residues: 1-390; Coverage: 100%)
Gene name: aauB
Sequence domains: Methylamine dehydrogenase heavy chain (MADH)
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Aralkylamine dehydrogenase light chain Chains: B, E, G, I
Molecule details ›
Chains: B, E, G, I
Length: 135 amino acids
Theoretical weight: 14.52 KDa
Source organism: Alcaligenes faecalis
UniProt:
  • Canonical: P84887 (Residues: 48-182; Coverage: 100%)
Gene name: aauA
Sequence domains: Methylamine dehydrogenase, L chain
Structure domains: Methylamine/Aralkylamine dehydrogenase light chain
Azurin Chain: C
Molecule details ›
Chain: C
Length: 128 amino acids
Theoretical weight: 13.71 KDa
Source organism: Alcaligenes faecalis
UniProt:
  • Canonical: P00281 (Residues: 1-128; Coverage: 100%)
Sequence domains: Copper binding proteins, plastocyanin/azurin family
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

1 bound ligand:
Ligand CU 1 x CU
1 modified residue:
Ligand TRQ 4 x TRQ

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 97.505Å b: 124.612Å c: 189.874Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.188 0.249

Quick links

Citations

9 review citations

Metalloproteins containing cytochrome, iron-sulfur, or copper redox centers.
Liu et al. (2014)
8 more

1 mention without citation

RL-MLZerD: Multimeric protein docking using reinforcement learning.
Aderinwale et al. (2022)