› 2h4q

X-ray diffraction
2.1Å resolution

Crystal structure of a M-loop deletion variant of MENT in the cleaved conformation

Released:
DOI: 10.2210/pdb2h4q/pdb
PDB entry 2h4q coloured by chain, front view.
PDB entry 2h4q coloured by chain, side view.
PDB entry 2h4q coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
X-ray crystal structure of MENT: evidence for functional loop-sheet polymers in chromatin condensation.
McGowan S, Buckle AM, Irving JA, Ong PC, Bashtannyk-Puhalovich TA, Kan WT, Henderson KN, Bulynko YA, Popova EY, Smith AI, Bottomley SP, Rossjohn J, Grigoryev SA, Pike RN, Whisstock JC
EMBO J 25 3144-55 (2006)
PMID: 16810322

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero dodecamer
Assembly name:
PDBe Complex ID:
PDB-CPX-130726 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Heterochromatin-associated protein MENT Chain: A
Molecule details ›
Chain: A
Length: 382 amino acids
Theoretical weight: 43.95 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: O73790 (Residues: 1-369; Coverage: 83%)
Gene names: MENT, SERPINB10
Sequence domains: Serpin (serine protease inhibitor)
Structure domains:
Heterochromatin-associated protein MENT Chain: B
Molecule details ›
Chain: B
Length: 34 amino acids
Theoretical weight: 4.19 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: O73790 (Residues: 377-410; Coverage: 8%)
Gene names: MENT, SERPINB10

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELLIOTT GX-13
Spacegroup: R32
Unit cell:
a: 150.194Å b: 150.194Å c: 175.485Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.189 0.188 0.213
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Conformational pathology of the serpins: themes, variations, and therapeutic strategies.
Gooptu et al. (2009)
9 more

1 mention without citation

X-ray crystal structure of MENT: evidence for functional loop-sheet polymers in chromatin condensation.
McGowan et al. (2006)