› 2h56

X-ray diffraction
2.55Å resolution

Crystal structure of DNA-3-methyladenine glycosidase (10174367) from Bacillus halodurans at 2.55 A resolution

Released:
DOI: 10.2210/pdb2h56/pdb
PDB entry 2h56 coloured by chain, front view.
PDB entry 2h56 coloured by chain, side view.
PDB entry 2h56 coloured by chain, top view.
Source organism: Alkalihalobacillus halodurans
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191828 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-3-methyladenine glycosylase II Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 233 amino acids
Theoretical weight: 26.42 KDa
Source organism: Alkalihalobacillus halodurans
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KC25 (Residues: 1-221; Coverage: 100%)
Gene name: BH1749
Sequence domains: HhH-GPD superfamily base excision DNA repair protein
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 15 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P65
Unit cell:
a: 141.752Å b: 141.752Å c: 85.949Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.198 0.196 0.246
Expression system: Escherichia coli

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