› 2h5r

X-ray diffraction
1.6Å resolution

Crystal structure of mStrawberry at pH 10.5

Released:
DOI: 10.2210/pdb2h5r/pdb
PDB entry 2h5r coloured by chain, front view.
PDB entry 2h5r coloured by chain, side view.
PDB entry 2h5r coloured by chain, top view.
Source organism: Discosoma sp.
Primary publication:
Novel chromophores and buried charges control color in mFruits.
Shu X, Shaner NC, Yarbrough CA, Tsien RY, Remington SJ
Biochemistry 45 9639-47 (2006)
PMID: 16893165

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193622 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Red fluorescent protein drFP583 Chain: A
Molecule details ›
Chain: A
Length: 234 amino acids
Theoretical weight: 26.68 KDa
Source organism: Discosoma sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9U6Y8 (Residues: 7-221; Coverage: 96%)
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CRO 1 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P21
Unit cell:
a: 48.646Å b: 44.177Å c: 61.179Å
α: 90° β: 105.98° γ: 90°
R-values:
R R work R free
0.166 0.166 0.217
Expression system: Escherichia coli

Quick links

Citations

37 review citations

Fluorescent proteins and their applications in imaging living cells and tissues.
Chudakov et al. (2010)
36 more

1 mention without citation

Chromophore transformations in red fluorescent proteins.
Subach et al. (2012)