› 2h6b

X-ray diffraction
2.2Å resolution

Crystal structure of oxidized CprK in complex with o-chlorophenolacetic acid

Released:
DOI: 10.2210/pdb2h6b/pdb
PDB entry 2h6b coloured by chain, front view.
PDB entry 2h6b coloured by chain, side view.
PDB entry 2h6b coloured by chain, top view.
Source organism: Desulfitobacterium hafniense
Primary publication:
CprK crystal structures reveal mechanism for transcriptional control of halorespiration.
Joyce MG, Levy C, Gábor K, Pop SM, Biehl BD, Doukov TI, Ryter JM, Mazon H, Smidt H, van den Heuvel RH, Ragsdale SW, van der Oost J, Leys D
J Biol Chem 281 28318-25 (2006)
PMID: 16803881

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
homo octamer
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186020 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative transcription regulatory protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 250 amino acids
Theoretical weight: 28.4 KDa
Source organism: Desulfitobacterium hafniense
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8RPJ2 (Residues: 1-202; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand 3C4 2 x 3C4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: I222
Unit cell:
a: 104.437Å b: 112.185Å c: 119.495Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.183 0.229
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Thiol-based redox switches and gene regulation.
Antelmann et al. (2011)
3 more

3 mentions without citation

Evolution of allostery in the cyclic nucleotide binding module.
Kannan et al. (2007)
2 more