› 2h7o

X-ray diffraction
2Å resolution

Crystal structure of the Rho-GTPase binding domain of YpkA

Released:
DOI: 10.2210/pdb2h7o/pdb
PDB entry 2h7o coloured by chain, front view.
PDB entry 2h7o coloured by chain, side view.
PDB entry 2h7o coloured by chain, top view.
Source organism: Yersinia pseudotuberculosis
Primary publication:
Yersinia virulence depends on mimicry of host Rho-family nucleotide dissociation inhibitors.
Prehna G, Ivanov MI, Bliska JB, Stebbins CE
Cell 126 869-80 (2006)
PMID: 16959567

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170231 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein kinase YpkA Chain: A
Molecule details ›
Chain: A
Length: 303 amino acids
Theoretical weight: 34.45 KDa
Source organism: Yersinia pseudotuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q05608 (Residues: 434-732; Coverage: 41%)
Gene names: pYV0001, ypkA
Sequence domains: Rac1-binding domain
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P6522
Unit cell:
a: 60.009Å b: 60.009Å c: 402.022Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.209 0.208 0.237
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

34 review citations

Regulation of small GTPases by GEFs, GAPs, and GDIs.
Cherfils et al. (2013)
33 more

5 mentions without citation

Protein-protein docking benchmark version 3.0.
Hwang et al. (2008)
4 more