› 2h8o

X-ray diffraction
1.6Å resolution

The 1.6A crystal structure of the geranyltransferase from Agrobacterium tumefaciens

Released:
DOI: 10.2210/pdb2h8o/pdb
PDB entry 2h8o coloured by chain, front view.
PDB entry 2h8o coloured by chain, side view.
PDB entry 2h8o coloured by chain, top view.
Source organism: Agrobacterium fabrum str. C58
Entry authors: Zhang R, Xu X, Gu J, Savchenko A, Edwards A, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
Geranyl diphosphate + isopentenyl diphosphate = diphosphate + (2E,6E)-farnesyl diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181445 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Geranyltranstransferase Chain: A
Molecule details ›
Chain: A
Length: 335 amino acids
Theoretical weight: 35.82 KDa
Source organism: Agrobacterium fabrum str. C58
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7CWE5 (Residues: 1-304; Coverage: 100%)
Gene names: Atu2721, ispA
Sequence domains: Polyprenyl synthetase
Structure domains: Farnesyl Diphosphate Synthase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21212
Unit cell:
a: 63.341Å b: 79.807Å c: 51.85Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.168 0.188
Expression system: Escherichia coli BL21(DE3)

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