› 2h9f

X-ray diffraction
1.95Å resolution

CRYSTAL STRUCTURE OF a PrpF family methylaconitate isomerase (PA0793) FROM PSEUDOMONAS AERUGINOSA AT 1.95 A RESOLUTION

Released:
DOI: 10.2210/pdb2h9f/pdb
PDB entry 2h9f coloured by chain, front view.
PDB entry 2h9f coloured by chain, side view.
PDB entry 2h9f coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191415 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-methylaconitate cis-trans isomerase PrpF Chain: A
Molecule details ›
Chain: A
Length: 396 amino acids
Theoretical weight: 42.28 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9I5E5 (Residues: 1-395; Coverage: 100%)
Gene name: PA0793
Sequence domains: PrpF protein
Structure domains: Diaminopimelate Epimerase; Chain A, domain 1

Ligands and Environments

4 bound ligands:
Ligand CO 1 x CO
Ligand CL 2 x CL
Ligand SO4 4 x SO4
Ligand GOL 2 x GOL
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL1-5
Spacegroup: I4122
Unit cell:
a: 148.69Å b: 148.69Å c: 141.085Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.168 0.196
Expression system: Escherichia coli

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