› 2h9w

X-ray diffraction
1.82Å resolution

Green fluorescent protein ground states: the influence of a second protonation site near the chromophore

Released:
DOI: 10.2210/pdb2h9w/pdb
PDB entry 2h9w coloured by chain, front view.
PDB entry 2h9w coloured by chain, side view.
PDB entry 2h9w coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Green fluorescent protein ground states: the influence of a second protonation site near the chromophore.
Bizzarri R, Nifosì R, Abbruzzetti S, Rocchia W, Guidi S, Arosio D, Garau G, Campanini B, Grandi E, Ricci F, Viappiani C, Beltram F
Biochemistry 46 5494-504 (2007)
PMID: 17439158

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 238 amino acids
Theoretical weight: 27.14 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 2-237; Coverage: 99%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
2 modified residues:
Ligand CRO 1 x CRO
Ligand CSD 1 x CSD

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P212121
Unit cell:
a: 51.886Å b: 62.933Å c: 71.035Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.166 0.186
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Green fluorescent protein based pH indicators for in vivo use: a review.
Bizzarri et al. (2009)
4 more