› 2han

X-ray diffraction
1.95Å resolution

Structural basis of heterodimeric ecdysteroid receptor interaction with natural response element hsp27 gene promoter

Released:
DOI: 10.2210/pdb2han/pdb
PDB entry 2han coloured by chain, front view.
PDB entry 2han coloured by chain, side view.
PDB entry 2han coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Novel DNA-binding element within the C-terminal extension of the nuclear receptor DNA-binding domain.
Jakób M, Kołodziejczyk R, Orłowski M, Krzywda S, Kowalska A, Dutko-Gwóźdź J, Gwóźdź T, Kochman M, Jaskólski M, Ozyhar A
Nucleic Acids Res 35 2705-18 (2007)
PMID: 17426125

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115332 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Protein ultraspiracle Chain: A
Molecule details ›
Chain: A
Length: 93 amino acids
Theoretical weight: 10.82 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: P20153 (Residues: 94-179; Coverage: 17%)
Gene names: CG4380, Cf1, NR2B4, usp
Sequence domains: Zinc finger, C4 type (two domains)
Structure domains: Erythroid Transcription Factor GATA-1, subunit A
Ecdysone receptor Chain: B
Molecule details ›
Chain: B
Length: 119 amino acids
Theoretical weight: 13.52 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: P34021 (Residues: 256-365; Coverage: 13%)
Gene names: CG1765, EcR, NR1H1
Sequence domains: Zinc finger, C4 type (two domains)
Structure domains: Erythroid Transcription Factor GATA-1, subunit A
5'-D(*CP*AP*AP*GP*GP*GP*TP*TP*CP*AP*AP*TP*GP*CP*AP*CP*TP*TP*GP*T)-3' Chain: C
Molecule details ›
Chain: C
Length: 20 nucleotides
Theoretical weight: 6.15 KDa
5'-D(*GP*AP*CP*AP*AP*GP*TP*GP*CP*AP*TP*TP*GP*AP*AP*CP*CP*CP*TP*T)-3' Chain: D
Molecule details ›
Chain: D
Length: 20 nucleotides
Theoretical weight: 6.12 KDa

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P21
Unit cell:
a: 46.716Å b: 59.79Å c: 65.179Å
α: 90° β: 106.7° γ: 90°
R-values:
R R work R free
0.182 0.18 0.217
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Ecdysteroid hormone action.
Spindler et al. (2009)
3 more

9 mentions without citation

The retinoid X receptors and their ligands.
Dawson et al. (2012)
8 more