› 2hax

X-ray diffraction
1.29Å resolution

Crystal structure of Bacillus caldolyticus cold shock protein in complex with hexathymidine

Released:
DOI: 10.2210/pdb2hax/pdb
PDB entry 2hax coloured by chain, front view.
PDB entry 2hax coloured by chain, side view.
PDB entry 2hax coloured by chain, top view.
Source organism: [Bacillus] caldolyticus
Primary publication:
Common mode of DNA binding to cold shock domains. Crystal structure of hexathymidine bound to the domain-swapped form of a major cold shock protein from Bacillus caldolyticus.
Max KE, Zeeb M, Bienert R, Balbach J, Heinemann U
FEBS J 274 1265-79 (2007)
PMID: 17266726

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115334 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Cold shock protein CspB Chains: A, B
Molecule details ›
Chains: A, B
Length: 66 amino acids
Theoretical weight: 7.34 KDa
Source organism: [Bacillus] caldolyticus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P41016 (Residues: 1-66; Coverage: 100%)
Gene name: cspB
Sequence domains: 'Cold-shock' DNA-binding domain
Structure domains:
5'-D(*TP*TP*TP*TP*TP*T)-3' Chains: C, D
Molecule details ›
Chains: C, D
Length: 6 nucleotides
Theoretical weight: 1.78 KDa
Source organism: [Bacillus] caldolyticus
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand MPD 2 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P21212
Unit cell:
a: 74.34Å b: 64.89Å c: 31.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.132 0.13 0.162
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

4 review citations

Origins of specificity in protein-DNA recognition.
Rohs et al. (2010)
3 more

8 mentions without citation

Mechanisms of Lin28-mediated miRNA and mRNA regulation--a structural and functional perspective.
Mayr et al. (2013)
7 more