› 2hay

X-ray diffraction
2.11Å resolution

The Crystal Structure of the Putative NAD(P)H-Flavin Oxidoreductase from Streptococcus pyogenes M1 GAS

Released:
DOI: 10.2210/pdb2hay/pdb
PDB entry 2hay coloured by chain, front view.
PDB entry 2hay coloured by chain, side view.
PDB entry 2hay coloured by chain, top view.
Source organism: Streptococcus pyogenes serotype M1
Entry authors: Kim Y, Duggan E, Clancy S, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189450 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitroreductase domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 224 amino acids
Theoretical weight: 25.99 KDa
Source organism: Streptococcus pyogenes serotype M1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9A120 (Residues: 1-221; Coverage: 100%)
Gene name: SPy_0512
Sequence domains: Nitroreductase family
Structure domains: NADH Oxidase

Ligands and Environments

2 bound ligands:
Ligand SO4 5 x SO4
Ligand FMN 4 x FMN
1 modified residue:
Ligand MSE 32 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 60.205Å b: 91.874Å c: 175.693Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.175 0.232
Expression system: Escherichia coli BL21(DE3)

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