› 2hcm

X-ray diffraction
2Å resolution

Crystal structure of mouse putative dual specificity phosphatase complexed with zinc tungstate, New York Structural Genomics Consortium

Released:
DOI: 10.2210/pdb2hcm/pdb
PDB entry 2hcm coloured by chain, front view.
PDB entry 2hcm coloured by chain, side view.
PDB entry 2hcm coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural genomics of protein phosphatases.
Almo SC, Bonanno JB, Sauder JM, Emtage S, Dilorenzo TP, Malashkevich V, Wasserman SR, Swaminathan S, Eswaramoorthy S, Agarwal R, Kumaran D, Madegowda M, Ragumani S, Patskovsky Y, Alvarado J, Ramagopal UA, Faber-Barata J, Chance MR, Sali A, Fiser A, Zhang ZY, Lawrence DS, Burley SK
J Struct Funct Genomics 8 121-40 (2007)
PMID: 18058037

Function and Biology Details

Reactions catalysed: 
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183910 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dual specificity phosphatase 28 Chain: A
Molecule details ›
Chain: A
Length: 164 amino acids
Theoretical weight: 17.59 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8BTR5 (Residues: 2-163; Coverage: 99%)
Gene name: Dusp28
Sequence domains: Dual specificity phosphatase, catalytic domain
Structure domains: Protein tyrosine phosphatase superfamily

Ligands and Environments

4 bound ligands:
Ligand WO4 1 x WO4
Ligand ZN 2 x ZN
Ligand NA 1 x NA
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P6122
Unit cell:
a: 53.207Å b: 53.207Å c: 174.485Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.151 0.15 0.196
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

24 review citations

Aiding and abetting roles of NOX oxidases in cellular transformation.
Block et al. (2012)
23 more

2 mentions without citation

Structural genomics of protein phosphatases.
Almo et al. (2007)
1 more