› 2hdf

X-ray diffraction
2.65Å resolution

Crystal structure of the Colicin I receptor Cir from E.coli

Released:
DOI: 10.2210/pdb2hdf/pdb
PDB entry 2hdf coloured by chain, front view.
PDB entry 2hdf coloured by chain, side view.
PDB entry 2hdf coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure of colicin I receptor bound to the R-domain of colicin Ia: implications for protein import.
Buchanan SK, Lukacik P, Grizot S, Ghirlando R, Ali MM, Barnard TJ, Jakes KS, Kienker PK, Esser L
EMBO J 26 2594-604 (2007)
PMID: 17464289

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148037 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Colicin I receptor Chain: A
Molecule details ›
Chain: A
Length: 639 amino acids
Theoretical weight: 71.76 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P17315 (Residues: 26-663; Coverage: 100%)
Gene names: JW2142, b2155, cir, cirA, feuA
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SR 1 x SR
Ligand OES 2 x OES
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2
Unit cell:
a: 90.593Å b: 84.336Å c: 99.458Å
α: 90° β: 109.16° γ: 90°
R-values:
R R work R free
0.241 0.239 0.291
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

TonB or not TonB: is that the question?
Krewulak et al. (2011)
10 more

10 mentions without citation

TonB-dependent transporters: regulation, structure, and function.
Noinaj et al. (2010)
9 more