› 2hdv

X-ray diffraction
2Å resolution

Crystal structure of the Src Homology-2 domain of the adapter protein SH2-B

Released:
DOI: 10.2210/pdb2hdv/pdb
PDB entry 2hdv coloured by chain, front view.
PDB entry 2hdv coloured by chain, side view.
PDB entry 2hdv coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural basis for phosphotyrosine recognition by the Src homology-2 domains of the adapter proteins SH2-B and APS.
Hu J, Hubbard SR
J Mol Biol 361 69-79 (2006)
PMID: 16824542

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187624 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SH2B adapter protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 111 amino acids
Theoretical weight: 12.28 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q91ZM2 (Residues: 519-627; Coverage: 14%)
Gene names: Sh2b1, Sh2bpsm1
Sequence domains: SH2 domain
Structure domains: SH2 domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P212121
Unit cell:
a: 36.05Å b: 57.87Å c: 99.47Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.21 0.24
Expression system: Escherichia coli

Quick links

Citations

10 review citations

SH2B1 (SH2-B) and JAK2: a multifunctional adaptor protein and kinase made for each other.
Maures et al. (2007)
9 more

1 mention without citation

'Hot' macromolecular crystals.
Koclega et al. (2009)