› 2he4

X-ray diffraction
1.45Å resolution

The crystal structure of the second PDZ domain of human NHERF-2 (SLC9A3R2) interacting with a mode 1 PDZ binding motif

Released:
DOI: 10.2210/pdb2he4/pdb
PDB entry 2he4 coloured by chain, front view.
PDB entry 2he4 coloured by chain, side view.
PDB entry 2he4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of PICK1 and other PDZ domains obtained with the help of self-binding C-terminal extensions.
Elkins JM, Papagrigoriou E, Berridge G, Yang X, Phillips C, Gileadi C, Savitsky P, Doyle DA
Protein Sci 16 683-94 (2007)
PMID: 17384233

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172337 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Na(+)/H(+) exchange regulatory cofactor NHE-RF2 Chain: A
Molecule details ›
Chain: A
Length: 90 amino acids
Theoretical weight: 9.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15599 (Residues: 147-230; Coverage: 25%)
Gene names: NHERF2, SLC9A3R2
Sequence domains: PDZ domain
Structure domains: Pdz3 Domain

Ligands and Environments

1 bound ligand:
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: C2
Unit cell:
a: 49.803Å b: 37.677Å c: 40.34Å
α: 90° β: 97.7° γ: 90°
R-values:
R R work R free
0.132 0.13 0.18
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Engineering of recombinant crystallization chaperones.
Koide S. (2009)
5 more

7 mentions without citation

Stereochemical determinants of C-terminal specificity in PDZ peptide-binding domains: a novel contribution of the carboxylate-binding loop.
Amacher et al. (2013)
6 more