› 2heo

X-ray diffraction
1.7Å resolution

General Structure-Based Approach to the Design of Protein Ligands: Application to the Design of Kv1.2 Potassium Channel Blockers.

Released:
DOI: 10.2210/pdb2heo/pdb
PDB entry 2heo coloured by chain, front view.
PDB entry 2heo coloured by chain, side view.
PDB entry 2heo coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structure-based secondary structure-independent approach to design protein ligands: Application to the design of Kv1.2 potassium channel blockers.
Magis C, Gasparini D, Lecoq A, Le Du MH, Stura E, Charbonnier JB, Mourier G, Boulain JC, Pardo L, Caruana A, Joly A, Lefranc M, Masella M, Menez A, Cuniasse P
J Am Chem Soc 128 16190-205 (2006)
PMID: 17165772

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115339 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Z-DNA-binding protein 1 Chains: A, D
Molecule details ›
Chains: A, D
Length: 67 amino acids
Theoretical weight: 7.44 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9QY24 (Residues: 8-70; Coverage: 15%)
Gene names: Dlm1, Zbp1
Sequence domains: Adenosine deaminase z-alpha domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
5'-D(*TP*CP*GP*CP*GP*CP*G)-3' Chains: B, E
Molecule details ›
Chains: B, E
Length: 7 nucleotides
Theoretical weight: 2.11 KDa
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P61
Unit cell:
a: 79.91Å b: 79.91Å c: 55.335Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.241 0.241 0.305
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

1 review citation

Vital significance of addition of "shape similarity" between solutes to shape complementarity for more precise molecular recognition in aqueous binary solvents.
Endo T. (2011)
10 other articles

5 mentions without citation

Notch receptor-ligand binding and activation: insights from molecular studies.
Chillakuri et al. (2012)
4 more