› 2het

X-ray diffraction
3Å resolution

Non-myristoylated bovine recoverin (truncated at C-terminus) with calcium bound to EF-hand 3

Released:
DOI: 10.2210/pdb2het/pdb
PDB entry 2het coloured by chain, front view.
PDB entry 2het coloured by chain, side view.
PDB entry 2het coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Tuning of a neuronal calcium sensor.
Weiergräber OH, Senin II, Zernii EY, Churumova VA, Kovaleva NA, Nazipova AA, Permyakov SE, Permyakov EA, Philippov PP, Granzin J, Koch KW
J Biol Chem 281 37594-602 (2006)
PMID: 17015448

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-149260 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Recoverin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 189 amino acids
Theoretical weight: 21.75 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P21457 (Residues: 2-190; Coverage: 94%)
Gene names: RCV1, RCVRN
Sequence domains: EF hand
Structure domains: EF-hand

Ligands and Environments

1 bound ligand:
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21
Unit cell:
a: 70.889Å b: 70.759Å c: 85.925Å
α: 90° β: 99.01° γ: 90°
R-values:
R R work R free
0.238 0.237 0.259
Expression system: Escherichia coli BL21

Quick links

Citations

4 review citations

Neuronal calcium sensor proteins: generating diversity in neuronal Ca2+ signalling.
Burgoyne RD. (2007)
3 more

4 mentions without citation

Double electron-electron resonance probes Ca²⁺-induced conformational changes and dimerization of recoverin.
Myers et al. (2013)
3 more