› 2hf1

X-ray diffraction
1.9Å resolution

Crystal structure of the putative Tetraacyldisaccharide-1-P 4-kinase from Chromobacterium violaceum. NESG target CvR39.

Released:
DOI: 10.2210/pdb2hf1/pdb
PDB entry 2hf1 coloured by chain, front view.
PDB entry 2hf1 coloured by chain, side view.
PDB entry 2hf1 coloured by chain, top view.
Source organism: Chromobacterium violaceum ATCC 12472
Entry authors: Vorobiev SM, Abashidze M, Seetharaman J, Chen CX, Jiang M, Cunningham K, Ma LC, Xiao R, Acton T, Montelione GT, Hunt JF, Tong L, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Reaction catalysed: 
ATP + (2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl phosphate) = ADP + (2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-4-O-phospho-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl phosphate)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domain:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-181822 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UPF0434 protein CV_3345 Chains: A, B
Molecule details ›
Chains: A, B
Length: 68 amino acids
Theoretical weight: 7.97 KDa
Source organism: Chromobacterium violaceum ATCC 12472
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7NSS5 (Residues: 1-60; Coverage: 100%)
Gene name: CV_3345
Sequence domains: Trm112p-like protein
Structure domains: N-terminal domain of TfIIb

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: C2221
Unit cell:
a: 27.517Å b: 93.854Å c: 87.219Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.233 0.233 0.284
Expression system: Escherichia coli

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