› 2hfd

Solution NMR

NMR structure of protein Hydrogenase-1 operon protein hyaE from Escherichia coli: Northeast Structural Genomics Consortium Target ER415

Released:
DOI: 10.2210/pdb2hfd/pdb
PDB entry 2hfd coloured by chain, ensemble of 20 models, front view.
PDB entry 2hfd coloured by chain, ensemble of 20 models, side view.
PDB entry 2hfd coloured by chain, ensemble of 20 models, top view.
Source organism: Escherichia coli
Primary publication:
Protein chaperones Q8ZP25_SALTY from Salmonella typhimurium and HYAE_ECOLI from Escherichia coli exhibit thioredoxin-like structures despite lack of canonical thioredoxin active site sequence motif.
Parish D, Benach J, Liu G, Singarapu KK, Xiao R, Acton T, Su M, Bansal S, Prestegard JH, Hunt J, Montelione GT, Szyperski T
J Struct Funct Genomics 9 41-9 (2008)
PMID: 19039680

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148804 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hydrogenase-1 operon protein HyaE Chain: A
Molecule details ›
Chain: A
Length: 140 amino acids
Theoretical weight: 15.97 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P19931 (Residues: 1-132; Coverage: 100%)
Gene names: JW0958, b0976, hyaE
Sequence domains: Hydrogenase-1 expression protein HyaE
Structure domains: Glutaredoxin

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: distance geometry simulated annealing molecular dynamics
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Structure and function of DsbA, a key bacterial oxidative folding catalyst.
Shouldice et al. (2011)
5 other articles

1 mention without citation

Protein chaperones Q8ZP25_SALTY from Salmonella typhimurium and HYAE_ECOLI from Escherichia coli exhibit thioredoxin-like structures despite lack of canonical thioredoxin active site sequence motif.
Parish et al. (2008)