› 2hfg

X-ray diffraction
2.61Å resolution

Crystal structure of hBR3 bound to CB3s-Fab

Released:
DOI: 10.2210/pdb2hfg/pdb
PDB entry 2hfg coloured by chain, front view.
PDB entry 2hfg coloured by chain, side view.
PDB entry 2hfg coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Synthetic anti-BR3 antibodies that mimic BAFF binding and target both human and murine B cells.
Lee CV, Hymowitz SG, Wallweber HJ, Gordon NC, Billeci KL, Tsai SP, Compaan DM, Yin J, Gong Q, Kelley RF, DeForge LE, Martin F, Starovasnik MA, Fuh G
Blood 108 3103-11 (2006)
PMID: 16840730

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-212387 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
CB3s Fab light chain (kappa) Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Ig-like domain-containing protein Chain: H
Molecule details ›
Chain: H
Length: 232 amino acids
Theoretical weight: 24.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6N093 (Residues: 5-70, 71-77, 78-194; Coverage: 46%)
Gene name: DKFZp686I04196
Sequence domains:
Structure domains: Immunoglobulins
Tumor necrosis factor receptor superfamily member 13C Chain: R
Molecule details ›
Chain: R
Length: 51 amino acids
Theoretical weight: 5.32 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96RJ3 (Residues: 7-54; Coverage: 26%)
Gene names: BAFFR, BR3, TNFRSF13C
Sequence domains: BAFF-R, TALL-1 binding

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: I213
Unit cell:
a: 146.408Å b: 146.408Å c: 146.408Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.195 0.252
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Antibody Structure and Function: The Basis for Engineering Therapeutics.
Chiu et al. (2019)
11 more

1 mention without citation

OptMAVEn--a new framework for the de novo design of antibody variable region models targeting specific antigen epitopes.
Li et al. (2014)