› 2hfs

X-ray diffraction
1.75Å resolution

Crystal structure of L. major mevalonate kinase

Released:
DOI: 10.2210/pdb2hfs/pdb
PDB entry 2hfs coloured by chain, front view.
PDB entry 2hfs coloured by chain, side view.
PDB entry 2hfs coloured by chain, top view.
Source organism: Leishmania major
Primary publication:
Structure, substrate recognition and reactivity of Leishmania major mevalonate kinase.
Sgraja T, Smith TK, Hunter WN
BMC Struct Biol 7 20 (2007)
PMID: 17397541

Function and Biology Details

Reaction catalysed: 
ATP + (R)-mevalonate = ADP + (R)-5-phosphomevalonate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175680 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mevalonate kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 332 amino acids
Theoretical weight: 36 KDa
Source organism: Leishmania major
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q4Q6K7 (Residues: 1-329; Coverage: 100%)
Gene name: LMJF_31_0560
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21
Unit cell:
a: 41.265Å b: 88.466Å c: 88.167Å
α: 90° β: 103.62° γ: 90°
R-values:
R R work R free
0.22 0.217 0.277
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Lipid metabolism in Trypanosoma brucei.
Smith et al. (2010)
1 more

4 mentions without citation

Structure, substrate recognition and reactivity of Leishmania major mevalonate kinase.
Sgraja et al. (2007)
3 more