› 2hg8

X-ray diffraction
1.8Å resolution

Crystal Structure of Cys315Ala mutant of human mitochondrial branched chain aminotransferase complexed with its substrate mimic, N-methyl leucine.

Released:
DOI: 10.2210/pdb2hg8/pdb
PDB entry 2hg8 coloured by chain, front view.
PDB entry 2hg8 coloured by chain, side view.
PDB entry 2hg8 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Human mitochondrial branched chain aminotransferase isozyme: structural role of the CXXC center in catalysis.
Yennawar NH, Islam MM, Conway M, Wallin R, Hutson SM
J Biol Chem 281 39660-71 (2006)
PMID: 17050531

Function and Biology Details

Reaction catalysed: 
L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127454 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Branched-chain-amino-acid aminotransferase, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 365 amino acids
Theoretical weight: 41.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O15382 (Residues: 28-392; Coverage: 93%)
Gene names: BCAT2, BCATM, BCT2, ECA40
Sequence domains: Amino-transferase class IV
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
2 bound ligands:
Ligand MLE 2 x MLE
Ligand ACY 1 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P21
Unit cell:
a: 59.179Å b: 109.278Å c: 59.449Å
α: 90° β: 97.23° γ: 90°
R-values:
R R work R free
0.252 0.252 0.282
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Metabolic and regulatory roles of leucine in neural cells.
Murín et al. (2008)
1 more