› 2hgc

Solution NMR

Solution NMR structure of the YjcQ protein from Bacillus subtilis. Northeast Structural Genomics target SR346.

Released:
DOI: 10.2210/pdb2hgc/pdb
PDB entry 2hgc coloured by chain, ensemble of 20 models, front view.
PDB entry 2hgc coloured by chain, ensemble of 20 models, side view.
PDB entry 2hgc coloured by chain, ensemble of 20 models, top view.
Source organism: Bacillus subtilis
Entry authors: Rossi P, Cort JR, Ho CK, Janjua H, Cunningham K, Ma L-C, Xiao R, Liu J, Baran M, Swapna GVT, Acton TB, Rost B, Kennedy MA, Montelione GT, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128320 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
YjcQ protein Chain: A
Molecule details ›
Chain: A
Length: 102 amino acids
Theoretical weight: 12.11 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: O31639 (Residues: 1-94; Coverage: 100%)
Gene names: BSU11950, yjcQ
Sequence domains: YjcQ protein
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: Noesy assignments made with iterative method using CS, 3J, Hyper (dihedral), and Dyana for simulated annealing MD. Converged structures are further refined using NIH-xplor followed by CNS in explicit water shell (Nielges) . Full lenght sequence was carried through the refinement protocol, the disordered regions and hexHIS tag are not reported. Structure based on 595 constraints, 249 long range, 163 dihedral constraints and 36 H-bond constraints.
Expression system: Escherichia coli

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