› 2hgz

X-ray diffraction
2.5Å resolution

Crystal structure of a p-benzoyl-L-phenylalanyl-tRNA synthetase

Released:
DOI: 10.2210/pdb2hgz/pdb
PDB entry 2hgz coloured by chain, front view.
PDB entry 2hgz coloured by chain, side view.
PDB entry 2hgz coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii
Primary publication:
Structural basis for the recognition of para-benzoyl-L-phenylalanine by evolved aminoacyl-tRNA synthetases.
Liu W, Alfonta L, Mack AV, Schultz PG
Angew Chem Int Ed Engl 46 6073-5 (2007)
PMID: 17628477

Function and Biology Details

Reaction catalysed: 
ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176482 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine--tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 306 amino acids
Theoretical weight: 34.93 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q57834 (Residues: 2-306; Coverage: 100%)
Gene names: MJ0389, tyrS
Sequence domains: tRNA synthetases class I (W and Y)
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PBF 1 x PBF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: P43212
Unit cell:
a: 101.639Å b: 101.639Å c: 71.466Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.21 0.27
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Adding new chemistries to the genetic code.
Liu et al. (2010)
3 more

3 mentions without citation

Photoinactivation of glutamate receptors by genetically encoded unnatural amino acids.
Klippenstein et al. (2014)
2 more