› 2hhl

X-ray diffraction
2.1Å resolution

Crystal structure of the human small CTD phosphatase 3 isoform 1

Released:
DOI: 10.2210/pdb2hhl/pdb
PDB entry 2hhl coloured by chain, front view.
PDB entry 2hhl coloured by chain, side view.
PDB entry 2hhl coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural genomics of protein phosphatases.
Almo SC, Bonanno JB, Sauder JM, Emtage S, Dilorenzo TP, Malashkevich V, Wasserman SR, Swaminathan S, Eswaramoorthy S, Agarwal R, Kumaran D, Madegowda M, Ragumani S, Patskovsky Y, Alvarado J, Ramagopal UA, Faber-Barata J, Chance MR, Sali A, Fiser A, Zhang ZY, Lawrence DS, Burley SK
J Struct Funct Genomics 8 121-40 (2007)
PMID: 18058037

Function and Biology Details

Reaction catalysed: 
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127350 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CTD small phosphatase-like protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 195 amino acids
Theoretical weight: 22.42 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O15194 (Residues: 82-267; Coverage: 67%)
Gene names: C3orf8, CTDSPL, NIF1, NIFL, SCP3, YA22
Sequence domains: NLI interacting factor-like phosphatase
Structure domains: HAD superfamily/HAD-like

Ligands and Environments

1 bound ligand:
Ligand KEG 3 x KEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P21
Unit cell:
a: 47.419Å b: 49.857Å c: 179.747Å
α: 90° β: 96.9° γ: 90°
R-values:
R R work R free
0.191 0.188 0.252
Expression system: Escherichia coli

Quick links

Citations

22 review citations

Aiding and abetting roles of NOX oxidases in cellular transformation.
Block et al. (2012)
21 more

7 mentions without citation

Structural genomics of protein phosphatases.
Almo et al. (2007)
6 more