› 2hi1

X-ray diffraction
2.3Å resolution

The structure of a putative 4-hydroxythreonine-4-phosphate dehydrogenase from Salmonella typhimurium.

Released:
DOI: 10.2210/pdb2hi1/pdb
PDB entry 2hi1 coloured by chain, front view.
PDB entry 2hi1 coloured by chain, side view.
PDB entry 2hi1 coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Entry authors: Cuff ME, Quartey P, Holzle D, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
(1a) 4-phospho-D-threonate + NAD(+) = 3-dehydro-4-phospho-D-erythronate + NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157713 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-threonate 4-phosphate dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 330 amino acids
Theoretical weight: 35.75 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P58718 (Residues: 1-327; Coverage: 100%)
Gene names: STM0163, pdxA2
Sequence domains: Pyridoxal phosphate biosynthetic protein PdxA
Structure domains: Isopropylmalate Dehydrogenase

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 77.66Å b: 92.892Å c: 101.328Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.187 0.266
Expression system: Escherichia coli BL21

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