› 2hi2

X-ray diffraction
2.3Å resolution

Crystal structure of native Neisseria gonorrhoeae Type IV pilin at 2.3 Angstroms Resolution

Released:
DOI: 10.2210/pdb2hi2/pdb
PDB entry 2hi2 coloured by chain, front view.
PDB entry 2hi2 coloured by chain, side view.
PDB entry 2hi2 coloured by chain, top view.
Source organism: Neisseria gonorrhoeae
Primary publication:
Type IV pilus structure by cryo-electron microscopy and crystallography: implications for pilus assembly and functions.
Craig L, Volkmann N, Arvai AS, Pique ME, Yeager M, Egelman EH, Tainer JA
Mol Cell 23 651-62 (2006)
PMID: 16949362

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136424 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Type IV major pilin protein PilE1 Chain: A
Molecule details ›
Chain: A
Length: 158 amino acids
Theoretical weight: 17.21 KDa
Source organism: Neisseria gonorrhoeae
UniProt:
  • Canonical: P02974 (Residues: 8-165; Coverage: 96%)
Gene name: pilE1
Sequence domains:
Structure domains: Glycoprotein, Type 4 Pilin

Ligands and Environments

Carbohydrate polymer : NEW Components: DT6, GLA
Carbohydrate polymer
2 bound ligands:
Ligand HTO 1 x HTO
Ligand OPE 1 x OPE
1 modified residue:
Ligand MEA 1 x MEA

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: C222
Unit cell:
a: 119.754Å b: 124.941Å c: 26.848Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.251 0.259 0.282

Quick links

Citations

56 review citations

X-ray solution scattering (SAXS) combined with crystallography and computation: defining accurate macromolecular structures, conformations and assemblies in solution.
Putnam et al. (2007)
55 more

21 mentions without citation

Exceptionally widespread nanomachines composed of type IV pilins: the prokaryotic Swiss Army knives.
Berry et al. (2015)
20 more