› 2hiv

X-ray diffraction
2.05Å resolution

ATP-dependent DNA ligase from S. solfataricus

Released:
DOI: 10.2210/pdb2hiv/pdb
PDB entry 2hiv coloured by chain, front view.
PDB entry 2hiv coloured by chain, side view.
PDB entry 2hiv coloured by chain, top view.
Source organism: Saccharolobus solfataricus
Primary publication:
A flexible interface between DNA ligase and PCNA supports conformational switching and efficient ligation of DNA.
Pascal JM, Tsodikov OV, Hura GL, Song W, Cotner EA, Classen S, Tomkinson AE, Tainer JA, Ellenberger T
Mol Cell 24 279-91 (2006)
PMID: 17052461

Function and Biology Details

Reaction catalysed: 
(1a) ATP + [DNA ligase]-L-lysine = [DNA ligase]-N(6)-(5'-adenylyl)-L-lysine + diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189101 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA ligase Chain: A
Molecule details ›
Chain: A
Length: 621 amino acids
Theoretical weight: 70 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q980T8 (Residues: 1-601; Coverage: 100%)
Gene names: SSO0189, lig
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 12.3.1
Spacegroup: C2221
Unit cell:
a: 119.206Å b: 169.77Å c: 77.31Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.209 0.254
Expression system: Escherichia coli

Quick links

Citations

28 review citations

PCNA, the maestro of the replication fork.
Moldovan et al. (2007)
27 more

7 mentions without citation

Archaeal Nucleic Acid Ligases and Their Potential in Biotechnology.
Chambers et al. (2015)
6 more