2hj1

X-ray diffraction
2.1Å resolution

Crystal structure of a 3D domain-swapped dimer of protein HI0395 from Haemophilus influenzae

Released:
Entry authors: Ramagopal UA, Patskovsky YV, Toro R, Almo SC, Burley SK, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-175730 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein RnfH Chains: A, B
Molecule details ›
Chains: A, B
Length: 97 amino acids
Theoretical weight: 11.23 KDa
Source organism: Haemophilus influenzae 86-028NP
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4QNE7 (Residues: 2-86; Coverage: 83%)
Gene names: NTHI0516, rnfH
Sequence domains: RnfH family Ubiquitin
Structure domains: RnfH-like

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A, NSLS BEAMLINE X29A
Spacegroup: P43212
Unit cell:
a: 74.381Å b: 74.381Å c: 117.027Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.215 0.231
Expression system: Escherichia coli