› 2hje

X-ray diffraction
1.7Å resolution

Crystal structure of Vibrio harveyi LuxQ periplasmic domain

Released:
DOI: 10.2210/pdb2hje/pdb
PDB entry 2hje coloured by chain, front view.
PDB entry 2hje coloured by chain, side view.
PDB entry 2hje coloured by chain, top view.
Source organism: Vibrio harveyi
Primary publication:
Ligand-induced asymmetry in histidine sensor kinase complex regulates quorum sensing.
Neiditch MB, Federle MJ, Pompeani AJ, Kelly RC, Swem DL, Jeffrey PD, Bassler BL, Hughson FM
Cell 126 1095-108 (2006)
PMID: 16990134

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156995 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Autoinducer 2 sensor kinase/phosphatase LuxQ Chain: A
Molecule details ›
Chain: A
Length: 221 amino acids
Theoretical weight: 24.87 KDa
Source organism: Vibrio harveyi
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P54302 (Residues: 52-271; Coverage: 26%)
Gene name: luxQ
Sequence domains: LuxQ, periplasmic
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand NI 4 x NI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P1
Unit cell:
a: 33.446Å b: 36.152Å c: 49.185Å
α: 82.18° β: 86.14° γ: 67.28°
R-values:
R R work R free
0.197 0.194 0.237
Expression system: Escherichia coli BL21

Quick links

Citations

41 review citations

Bacterial quorum-sensing network architectures.
Ng et al. (2009)
40 more

10 mentions without citation

Structure and signaling mechanism of Per-ARNT-Sim domains.
Möglich et al. (2009)
9 more