› 2hjg

X-ray diffraction
2.5Å resolution

The crystal structure of the B. subtilis YphC GTPase in complex with GDP

Released:
DOI: 10.2210/pdb2hjg/pdb
PDB entry 2hjg coloured by chain, front view.
PDB entry 2hjg coloured by chain, side view.
PDB entry 2hjg coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
The essential GTPase YphC displays a major domain rearrangement associated with nucleotide binding.
Muench SP, Xu L, Sedelnikova SE, Rice DW
Proc Natl Acad Sci U S A 103 12359-64 (2006)
PMID: 16894162

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156259 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTPase Der Chain: A
Molecule details ›
Chain: A
Length: 436 amino acids
Theoretical weight: 49.39 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P50743 (Residues: 1-436; Coverage: 100%)
Gene names: BSU22840, der, engA, yphC
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand GDP 2 x GDP
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX10.1
Spacegroup: P212121
Unit cell:
a: 62.711Å b: 65.045Å c: 110.61Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.215 0.274
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Structure-function relationships of the G domain, a canonical switch motif.
Wittinghofer et al. (2011)
4 more

8 mentions without citation

The universally conserved prokaryotic GTPases.
Verstraeten et al. (2011)
7 more