› 2hjh

X-ray diffraction
1.85Å resolution

Crystal Structure of the Sir2 deacetylase

Released:
DOI: 10.2210/pdb2hjh/pdb
PDB entry 2hjh coloured by chain, front view.
PDB entry 2hjh coloured by chain, side view.
PDB entry 2hjh coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Entry authors: Hall BE, Ellenberger TE

Function and Biology Details

Reaction catalysed: 
(1a) [protein]-N(6)-acetyl-L-lysine + NAD(+) = [protein]-N(6)-(1,1-(5-adenosylyl-alpha-D-ribose-1,2-di-O-yl)ethyl)-L-lysine + nicotinamide
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139137 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD-dependent histone deacetylase SIR2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 354 amino acids
Theoretical weight: 40.7 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P06700 (Residues: 209-562; Coverage: 63%)
Gene names: D2714, MAR1, SIR2, YDL042C
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand XYQ 2 x XYQ
Ligand NCA 2 x NCA
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 52.353Å b: 89.566Å c: 94.51Å
α: 90° β: 104.95° γ: 90°
R-values:
R R work R free
0.173 0.171 0.215
Expression system: Escherichia coli

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