› 2hka

X-ray diffraction
1.81Å resolution

Crystal structure of bovine NPC2 and cholesterol sulfate complex

Released:
DOI: 10.2210/pdb2hka/pdb
PDB entry 2hka coloured by chain, front view.
PDB entry 2hka coloured by chain, side view.
PDB entry 2hka coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Structural basis of sterol binding by NPC2, a lysosomal protein deficient in Niemann-Pick type C2 disease.
Xu S, Benoff B, Liou HL, Lobel P, Stock AM
J Biol Chem 282 23525-31 (2007)
PMID: 17573352

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160320 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NPC intracellular cholesterol transporter 2 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 130 amino acids
Theoretical weight: 14.63 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P79345 (Residues: 20-149; Coverage: 100%)
Gene name: NPC2
Sequence domains: ML domain
Structure domains: Immunoglobulin-like

Ligands and Environments

5 bound ligands:
Ligand NAG 3 x NAG
Ligand ACT 6 x ACT
Ligand SO4 3 x SO4
Ligand C3S 2 x C3S
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2
Unit cell:
a: 122.38Å b: 62.163Å c: 72.449Å
α: 90° β: 98.62° γ: 90°
R-values:
R R work R free
0.195 0.195 0.214

Quick links

Citations

42 review citations

Cellular cholesterol trafficking and compartmentalization.
Ikonen E. (2008)
41 more

24 mentions without citation

Soluble proteins of chemical communication: an overview across arthropods.
Pelosi et al. (2014)
23 more